Measurement of substrate thermal resistance using DNA denaturation temperature
نویسندگان
چکیده
منابع مشابه
1/f fluctuations of DNA temperature at thermal denaturation.
We theoretically investigated the temperature fluctuations of DNA close to denaturation and observed a strong enhancement of these fluctuations at the critical temperature. Although in a much lower frequency range, such a sharp increase was also reported in the recent experimental work of Nagapriya [Phys. Rev. Lett. 96, 038102 (2006)]. We showed that there is instead no enhancement of temperatu...
متن کاملDirect observation of large temperature fluctuations during DNA thermal denaturation.
In this Letter, we report direct measurement of large low frequency temperature fluctuations in double stranded DNA when it undergoes a denaturation transition. The fluctuation, which occurs only in the temperature range where the denaturation occurs, is several orders more than the expected equilibrium fluctuation. It is absent in single stranded DNA of the same sequence. The fluctuation at a ...
متن کاملThermal denaturation of A-DNA
The DNA molecule can take various conformational forms. Investigations focus mainly on the so-called ‘B-form’, schematically drawn in the famous paper by Watson and Crick [1]. This is the usual form of DNA in a biological environment and is the only form that is stable in an aqueous environment. Other forms, however, can teach us much about DNA. They have the same nucleotide base pairs for ‘bui...
متن کاملFast plasmid DNA sequencing using a thermal cycler and high temperature alkali denaturation.
This report describes a modification of the Chen and Seeburg plasmid sequencing procedure (2). Chen and Seeburg demonstrated that the collapse of closed covalent circular plasmid DNA into networked DNA favored doublestranded sequencing; however, the method was sensitive to genomic DNA and RNA contamination. Complete alkaline denaturation (not thermal) and RNA degradation were essential for high...
متن کاملMechanical denaturation of DNA: existence of a low temperature denaturation
Recent theoretical predictions on DNA mechanical separation induced by pulling forces are numerically tested within a model in which self-avoidance for DNA strands is fully taken into account. DNA strands are described by interacting pairs of self avoiding walks (SAW) which are pulled apart by a force applied at the two extremities. The whole phase diagram is spanned by extensive Monte Carlo (M...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Journal of Thermal Sciences
سال: 2010
ISSN: 1290-0729
DOI: 10.1016/j.ijthermalsci.2009.07.026